Pre-defense of the dissertation for the degree of Doctor of Philosophy (PhD) of Sandybayeva Sandugash Kalzhankyzy

On December 4, 2024, a pre-defense of the dissertation for the degree of Doctor of Philosophy (PhD) of Sandybayeva Sandugash Kalzhankyzy on the topic “Isolation and screening of potential strains of cyanobacteria-producers of valuable pigments for obtaining biologically active additives” on the specility "8D05105 - Biotechnology".

The work is devoted to isolation and study of active strains of cyanobacteria-producers of valuable pigments from various extreme aquatic ecosystems for obtaining biologically active additives.

Regarding obtained results, the algological composition and diversity of phototrophic microorganisms from the hot springs of Chundzha in the Uyghur District of the Almaty region were studied. As a result, eight axenic cultures of cyanobacteria were isolated, and their morphological and cultural properties were analyzed. A screening of the isolated cultures was conducted to evaluate their productivity and the accumulation of bioactive compounds (proteins, lipids, fatty acids, etc.). Qualitative and quantitative pigment analyses were performed on the cells of selected active strains, including Oscillatoria subbrevis CZS 2201, Phormidium ambiguum CZS 2205, Nostoc calcicola TSZ 2203, and Synechococcus sp. CZS 2204. Based on laboratory studies, a glycosylated carotenoid, myxoxanthophyll, with a purity of at least 93%, was obtained from the biomass of Oscillatoria subbrevis CZS 2201 for the first time.

The immunomodulatory effect of extracts from active complexes derived from cyanobacterial strains was evaluated on the growth and proliferation of tumor cell lines MiaPaCa2 (pancreatic carcinoma), HepG2 (liver carcinoma), and K562 (human myelogenous leukemia), as well as on the proliferation and growth of immune-competent bone marrow cells in mice.

Biotechnology Department